Nanopore Sequencing
We provide long-read sequencing using PromethION instruments by Oxford Nanopore Tehnologies. We include base calling with any model and DNA methylation.
Bioinformatics
Let us analyze the data for you! We provide streamlined bioinformatics analysis for variant calling and differential DNA methylation using the latest tools.
Consulting
Want to get started with your own sequencing setup? Talk to Chris and learn what's needed to start sequencing on your own.
Our Services
Nanopore Sequencing (PromethION)
$2000 per flowcell (PromethION). What can you get for that? 90-120 Gb of sequence. Equivalent to 30-40X depth for a mammalian genome. DNA methylation calls included! You can divide up to 6 samples and barcode the output (e.g., 15-20 Gb each)
Genome-wide DNA Methylation
$0 (included in the price of sequencing). You get DNA methylation values at all 28 million CpG sites.
DNA Methylation Bioinformatics
$500 per 6 samples. You get DMLs/DMRs between 2 groups of up to 3 replicates each (1 rep minimum). A report with violin plots, bar plots, and sample correlations. Sequencing not included.
Global DNA Methylation (96 samples)
$2500 gets you global DNA Methylation values for up to 96 samples using genome skimming. The service also provides aggregate methylation levels at TSS and LINE and Alu transposons.
Genome Assembly
$6000 per species. Give us tissue and get a de novo assembled publishable genome. All animals accepted.
Consulting
Want to set up your own sequencing center? How about a portable field lab for sequencing? Need hands-on assistance in the field or the lab? Need software help? Talk to our experts. We consult by zoom, in-person, and across the globe.
Our Prices
Contact us by email for more details
90-120 Gb of Nanopore Sequencing
$2000
One PromethION flow cell run on a P2 Solo instrument. Base calling in 'super accuracy' mode with 5mC detection included. QC metrics included. Up to 6 samples barcoded per flow cell. DNA will be sheared to 5-10 kb and aligned to the genome of your choice. Delivery is QC, Bam, and Fastq files. 2 ug per sample required.
Genome Skimming (96 samples)
$2500
Global DNA methylation values for up to 96 samples. Included is the global 5mC DNA methylation percentage, aggregate TSS percentage, and aggregate LINE and Alu transposons. Data has other uses as well. 500 ng per sample required.
Hydroxymethylation calling (5hmC)
$300
For any sequencing service, we can perform 5'Hydroxymethylation base calling (5hmC) as an optional add-on per flow cell. We recommend this for neuronally derived tissues in all species.
DNA extraction from tissue
$20
Qiagen MagAttract HMW tissue extraction.
Priority Service
$3000
Our current rate is 6 samples per week with a 3 week lead time. For additional costs, your samples can gain priority and our capacity can increase to 12 samples per week.
Field Service
$TBD
Our skilled technicians will travel to the exotic location of your choice and perform on-the-spot sequencing in the field for you! Moon suits not included.
Standard Methylation Pipeline
$500
Methylkit pipeline for differential DNA methylation at CpG sites (DMLs) and regions (DMRs). Genome-wide 5mC violin plots, Bar plots for hypo/hyper DMLs/DMRs. Methylartist plot for every DMR. Regional enrichment (e.g., promoters, introns, exons, CpG islands, shores, etc). Requires >10X depth of Nanopore sequencing. 6 sample max. 2-group pairwise comparison only. See demo report.
Enhanced Methylation Pipeline
$1000
Some experimental designs require more complex analysis if they have more than 2 groups or nested designs. This pipeline provides DML/DMRs for these types of experiments. Discuss prior to purchase.
Genome Assembly (with sequencing)
$6000
You provide the tissue or DNA and we provide a publishable assembly. This service includes 2 promethION flow cells and typically provides >60X coverage for a mammal size genome. Assembled to contig level only. BUSCO scores included. Decontamination included. Methylome included.
Genome Assembly (without sequencing)
$3000
Same as above without us sequencing. You provide the fastq files, we perform the assembly. Long-reads preferred.
Custom Bioinformatics
$100/hr
Discuss your needs and we will provide a time estimate for your project.
Field Kit Advising
$3000$
Guide and up-to-date equipment list to start your own field sequencing lab. Laptop recommendations, equipment, and software pipeline described. This is a blueprint, not an implementation.
Lab Sequencing Startup Advising
$5000
Guide and up-to-date equipment list to start your own sequencing center in your lab using a PromethION P2 Solo. Custom computer build list, network build list, and storage array designs included.
Ready-to-Go Complete Sequencing Center
$40,000
Implemented Lab Sequencing Startup. Includes custom built computer capable of live basecalling a P2 Solo, 100 Tb storage array, and networking equipment, P2 sold directly by ONT. Includes library prep training and data analysis training.
T-shirts
$30
Show your pride for Decorative Genomics!
Applications
Successful projects in my lab
Conservation
Eco-Genomics
Field sequencing of DNA: ● Identify new subspecies● Population genomics ● Non-invasive sampling
Pathogens
Surveillance Genomics
Nanopore sequencing can:● Detect tick-borne pathogens● Detect commensal bacteria● Detect human viruses
Agriculture
Pest Genomics
Genomics for crop pests has:● Sequenced the genome of the soybean midge● Detected GMOs
Clinical Health
Disease Genomics
Personal Genomics in the clinic can:● Identify pathogens & resistance● ID mutations in newborns● Exomes/Genomes/Epigenetics
Fast turnaround
Why wait?
Bioinformatics
We have computers!
Field Capable
Sequence that bug!
Raw Data
Get all your stuff.
About Us
Who What Why?
About Us
I am a university professor with 20+ years of research involving DNA and epigenetics. My lab routinely sequences DNA from species across the vertebrate radiation and measures DNA methylation, hydroxmethylation, along with sequence variants. Let me help you in your next sequencing project!
Purpose
I established this company to bring speedy long-read sequencing and data analysis to those who don't want to wait for core services. We also provide bioinformatics reports for long-read sequencing with an emphasis on DNA methylation. We also offer consulting services to set up your own in-house sequencing setups for lab or field.
Fixed Price
Tired of "Contact Us" to see a price? Not here!
Our prices are right out front! We keep the options limited to keep costs down and fast turnaround. Each service is a bundle of fixed options that our company regularly performs with high accuracy. There are plenty of general sequencing services companies out there. Our niche is for sequencing and data analysis for the ONT platform.
Our Team
The experts behind the name
Have Any Questions? Call or email memoc.scimonegoced%40kluafc
Experience