Standard Methylation Pipeline
Differential DNA methylation analysis with Methylkit
Read Statistics and Plots
Plots will be generated for each sample including ● Methylation level histograms● Read coverage histograms● 5mC correlation plots for all samples● Violin plots of methylation for all samples● PCA plot
Differential Methylation (DMLs and DMRs)
Plots will be generated at various cutoff levels for hypo- and hyper-methylated regions as generated by Methylkit.
Regional enrichment
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Visualized regions
We use Methylartist to visualize all DMRs detected at your cutoff level of choice.
Deliverables
All files associated with the analysis will be delivered. You will receive the complete list of DMLs and DMRs with adjusted p-values and methylation scores. A powerpoint containing all the plots shown here, and a folder of all DMRs visualized with Methylartist.